5W7Q
Crystal Structure of Reconstructed Bacterial Elongation Factor Node 168
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-22 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.476, 104.046, 43.833 |
Unit cell angles | 90.00, 111.27, 90.00 |
Refinement procedure
Resolution | 30.537 - 2.500 |
R-factor | 0.2002 |
Rwork | 0.197 |
R-free | 0.24720 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.623 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 6.780 | 2.500 |
Rmerge | 0.099 | 0.066 | 0.435 |
Total number of observations | 47115 | ||
Number of reflections | 10877 | ||
<I/σ(I)> | 9.7 | ||
Completeness [%] | 92.2 | 90 | 82.4 |
Redundancy | 4.3 | 4.2 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% Peg 10K, 100mM Na Hepes |