5W7E
Murine acyloxyacyl hydrolase (AOAH), S262A mutant, with dimyristoyl phosphatidylcholine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-17 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.425, 85.373, 93.398 |
| Unit cell angles | 90.00, 103.64, 90.00 |
Refinement procedure
| Resolution | 40.073 - 1.830 |
| R-factor | 0.1578 |
| Rwork | 0.157 |
| R-free | 0.19780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.893 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.830 |
| Number of reflections | 106148 |
| <I/σ(I)> | 19.9 |
| Completeness [%] | 100.0 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1 mM Triton X-100, 2 mM dimyristoyl phosphatidylcholine; 100 mM sodium MES pH 6, 15% PEG 20000 |
