5W7D
Murine acyloxyacyl hydrolase (AOAH), S262A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-08-01 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.527, 98.590, 73.488 |
| Unit cell angles | 90.00, 99.68, 90.00 |
Refinement procedure
| Resolution | 38.703 - 1.750 |
| R-factor | 0.1539 |
| Rwork | 0.152 |
| R-free | 0.18150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.032 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.750 |
| Number of reflections | 66110 |
| <I/σ(I)> | 24.4 |
| Completeness [%] | 100.0 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM sodium acetate pH 4.6, 15% PEG 20000 |
