5W76
Crystal Structure of Reconstructed Bacterial Elongation Factor Node 168
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-21 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.326, 73.357, 114.377 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.447 - 2.152 |
R-factor | 0.192 |
Rwork | 0.190 |
R-free | 0.22340 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.230 |
High resolution limit [Å] | 2.150 | 4.630 | 2.150 |
Rmerge | 0.081 | 0.068 | 0.223 |
Total number of observations | 124793 | ||
Number of reflections | 23511 | ||
<I/σ(I)> | 18.8 | ||
Completeness [%] | 87.3 | 99.7 | 65.6 |
Redundancy | 5.3 | 5.5 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 40% PEG 200 and 0.1 M HEPES pH 7.0 |