5W76

Crystal Structure of Reconstructed Bacterial Elongation Factor Node 168

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-21
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	57.326, 73.357, 114.377
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.447 - 2.152
R-factor	0.192
Rwork	0.190
R-free	0.22340
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.230
High resolution limit [Å]	2.150	4.630	2.150
Rmerge	0.081	0.068	0.223
Total number of observations	124793
Number of reflections	23511
<I/σ(I)>	18.8
Completeness [%]	87.3	99.7	65.6
Redundancy	5.3	5.5	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	40% PEG 200 and 0.1 M HEPES pH 7.0