5W75

Crystal Structure of Reconstructed Bacterial Elongation Factor Node 168

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-27
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	91.417, 173.420, 208.178
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.705 - 2.298
R-factor	0.1941
Rwork	0.194
R-free	0.22020
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.380
High resolution limit [Å]	2.300	4.950	2.300
Rmerge	0.081	0.046	0.470
Total number of observations	858145
Number of reflections	145229
<I/σ(I)>	13.2
Completeness [%]	98.4	99.4	93.5
Redundancy	5.9	6.1	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	1.35 M ammonium sulfate and 0.1 M HEPES pH 7.5