5W70
X-ray Structure of RbmB from Streptomyces ribosidificus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-02-03 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 101.072, 101.072, 160.004 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.18497 |
| Rwork | 0.182 |
| R-free | 0.23322 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c7t |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.083 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.092 | 0.375 |
| Number of reflections | 48736 | 6185 |
| <I/σ(I)> | 10.3 | 2.8 |
| Completeness [%] | 99.2 | 98.2 |
| Redundancy | 8.3 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 24-26% PEG-3350, 100 mM MgCl2, 100 mM MOPS, 1 mM PLP, 5 mM 2-DOS |
