5W6T
Crystal structure of the A/Puerto Rico/8/1934 (H1N1) influenza virus hemagglutinin in complex with cyclic peptide CP151070 (P7)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2015-12-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0331 |
Spacegroup name | I 21 3 |
Unit cell lengths | 164.331, 164.331, 164.331 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.080 - 2.590 |
R-factor | 0.221 |
Rwork | 0.219 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ru7 |
RMSD bond length | 0.009 |
RMSD bond angle | 0.966 |
Data reduction software | HKL-2000 (712) |
Data scaling software | HKL-2000 (712) |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.630 |
High resolution limit [Å] | 2.590 | 2.590 |
Number of reflections | 21313 | |
<I/σ(I)> | 14.8 | 1.2 |
Completeness [%] | 92.2 | 90.9 |
Redundancy | 3.1 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 277 | HA: 10 mg/ml 1M lithium chloride 20 % w/v PEG 6000 0.1M MES, pH=5.3 |