5W5E
Re-refinement of the pyocin tube structure
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | KODAK SO-163 FILM |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3.5 |
| Software | PHENIX,RosettaCM |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
