5W4U
Pol II elongation complex with an N6-methyladenine-containing template
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 167.210, 221.990, 192.700 |
Unit cell angles | 90.00, 100.58, 90.00 |
Refinement procedure
Resolution | 81.021 - 3.600 |
R-factor | 0.2286 |
Rwork | 0.227 |
R-free | 0.25910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nvz |
RMSD bond length | 0.002 |
RMSD bond angle | 0.514 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 95.800 |
High resolution limit [Å] | 3.600 |
Number of reflections | 79907 |
<I/σ(I)> | 5 |
Completeness [%] | 100.0 |
Redundancy | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 50mM dioxane, 10mM DTT, 10-12.5% PEG6000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |