5W4Q
Structure of the R18A/E28A mutant of the HIV-1 capsid protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.033203 |
Spacegroup name | P 6 |
Unit cell lengths | 92.055, 92.055, 58.253 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.030 - 2.285 |
R-factor | 0.208 |
Rwork | 0.206 |
R-free | 0.24520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | pdbid 4XFX |
RMSD bond length | 0.003 |
RMSD bond angle | 0.564 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.31) |
Phasing software | PHASER (2.7.17) |
Refinement software | PHENIX ((1.11.1-2575_1692)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.034 | 47.030 | 2.360 |
High resolution limit [Å] | 2.280 | 8.850 | 2.280 |
Rmerge | 0.075 | 0.039 | 0.930 |
Rmeas | 0.083 | 0.044 | 1.026 |
Rpim | 0.035 | 0.020 | 0.428 |
Number of reflections | 12728 | 1246 | |
<I/σ(I)> | 13.6 | ||
Completeness [%] | 98.6 | 99.6 | 98.1 |
Redundancy | 5.6 | 5.3 | 5.6 |
CC(1/2) | 0.996 | 0.995 | 0.579 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | PEG3350, NaI, Sodium cacodylate, Glycerol |