5W4P
Structure of the E28A mutant of the HIV-1 capsid protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.033203 |
Spacegroup name | P 6 |
Unit cell lengths | 92.672, 92.672, 57.969 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.336 - 2.190 |
R-factor | 0.2146 |
Rwork | 0.213 |
R-free | 0.24580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | pdbid 4XFX |
RMSD bond length | 0.002 |
RMSD bond angle | 0.481 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.31) |
Phasing software | PHASER (2.7.17) |
Refinement software | PHENIX ((1.11.1-2575_1692)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.990 | 46.990 | 2.260 |
High resolution limit [Å] | 2.190 | 9.030 | 2.190 |
Rmerge | 0.056 | 0.035 | 0.639 |
Rmeas | 0.062 | 0.040 | 0.711 |
Rpim | 0.026 | 0.018 | 0.308 |
Number of reflections | 14722 | 1236 | |
<I/σ(I)> | 16.2 | ||
Completeness [%] | 99.8 | 98.9 | 98.6 |
Redundancy | 5.6 | 5.3 | 5.1 |
CC(1/2) | 0.997 | 0.994 | 0.779 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | PEG3350, NaI, Sodium cacodylate, Glycerol |