5W4M
Crystal structure of Streptococcus dysgalactiae SHP pheromone receptor Rgg2(C45S)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL14-1 |
Synchrotron site | SSRL |
Beamline | BL14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-25 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.1808 |
Spacegroup name | I 4 |
Unit cell lengths | 174.982, 174.982, 57.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.745 - 2.388 |
R-factor | 0.1949 |
Rwork | 0.193 |
R-free | 0.22420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yv6 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.445 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.440 |
High resolution limit [Å] | 2.388 | 6.510 | 2.388 |
Rmerge | 0.106 | 0.067 | 0.828 |
Rmeas | 0.115 | 0.072 | 0.891 |
Rpim | 0.042 | 0.027 | 0.327 |
Number of reflections | 34868 | 1725 | |
<I/σ(I)> | 13.1 | 3.4 | |
Completeness [%] | 100.0 | 99.6 | 100 |
Redundancy | 7.5 | 7.2 | 7.4 |
CC(1/2) | 0.996 | 0.807 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.75 | 293 | 350mM KSCN, 100mM Bis-Tris propane pH 7.75, 18% PEG 3350 |