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5W4K

Crystal structure of the Thermus thermophilus 70S ribosome in complex with Klebsazolicin and bound to mRNA and A-, P- and E-site tRNAs at 2.7A resolution

This is a non-PDB format compatible entry.
Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 24-ID-C
Synchrotron siteAPS
Beamline24-ID-C
Temperature [K]100
Detector technologyPIXEL
Collection date2016-07-03
DetectorDECTRIS PILATUS 6M-F
Wavelength(s)0.97950
Spacegroup nameP 21 21 21
Unit cell lengths209.640, 449.060, 622.010
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution155.503 - 2.700
R-factor0.2091
Rwork0.207
R-free0.25230
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.005
RMSD bond angle0.888
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwarePHASER
Refinement softwarePHENIX (1.8.2)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]155.503155.5032.770
High resolution limit [Å]2.70012.0702.700
Rmerge0.1590.0511.377
Rmeas0.1780.0571.605
Total number of observations7814032
Number of reflections157343018587108609
<I/σ(I)>9.230.960.97
Completeness [%]99.399.293
Redundancy4.9664.8093.596
CC(1/2)0.9910.9940.239
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION7.62920.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME

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