5W4K

Crystal structure of the Thermus thermophilus 70S ribosome in complex with Klebsazolicin and bound to mRNA and A-, P- and E-site tRNAs at 2.7A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-03
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	209.640, 449.060, 622.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	155.503 - 2.700
R-factor	0.2091
Rwork	0.207
R-free	0.25230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.888
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	155.503	155.503	2.770
High resolution limit [Å]	2.700	12.070	2.700
Rmerge	0.159	0.051	1.377
Rmeas	0.178	0.057	1.605
Total number of observations	7814032
Number of reflections	1573430	18587	108609
<I/σ(I)>	9.2	30.96	0.97
Completeness [%]	99.3	99.2	93
Redundancy	4.966	4.809	3.596
CC(1/2)	0.991	0.994	0.239

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME