5W4G

Importin binding to NLS peptide of DNA polymerase lambda

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-11
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	78.195, 89.747, 98.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.799 - 2.038
R-factor	0.1721
Rwork	0.171
R-free	0.19980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5e6q
RMSD bond length	0.004
RMSD bond angle	0.689
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.038	2.100
Number of reflections	41954
<I/σ(I)>	13.6
Completeness [%]	96.9
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1 M Bis Tris Propane 1.4 M Ammonium Sulfate