5W4D
C. japonica N-domain, Selenomethionine mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-10-07 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97857 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 133.300, 94.800, 72.500 |
Unit cell angles | 90.00, 91.40, 90.00 |
Refinement procedure
Resolution | 48.466 - 1.599 |
R-factor | 0.1577 |
Rwork | 0.157 |
R-free | 0.18980 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.004 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.466 | 1.656 |
High resolution limit [Å] | 1.599 | 1.599 |
Rmerge | 0.047 | 0.877 |
Rpim | 0.018 | 0.348 |
Number of reflections | 227476 | 11239 |
<I/σ(I)> | 24.44 | 2.3 |
Completeness [%] | 97.1 | 95.16 |
Redundancy | 7.6 | 7.2 |
CC(1/2) | 0.999 | 0.740 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100 mM Imidazole pH 7.5, 40-45% PEG 400, 1 mM TCEP pH 7.4 |