5W37
Crystal structure of mutant CJ YCEI protein (CJ-N182C) for nanotechnology applications
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2016-12-02 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.0 |
Spacegroup name | P 6 2 2 |
Unit cell lengths | 178.429, 178.429, 50.583 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.120 - 2.520 |
R-factor | 0.2172 |
Rwork | 0.215 |
R-free | 0.25780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5w17 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.935 |
Data reduction software | XDS (20161101) |
Data scaling software | SCALA (3.3.22) |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 38.236 | 38.236 | 2.660 |
High resolution limit [Å] | 2.520 | 7.970 | 2.520 |
Rmerge | 0.024 | 3.000 | |
Rmeas | 0.141 | 0.024 | 3.090 |
Rpim | 0.031 | 0.006 | 0.733 |
Total number of observations | 336425 | ||
Number of reflections | 16542 | ||
<I/σ(I)> | 22.8 | 20 | 0.3 |
Completeness [%] | 99.9 | 99.1 | 99.5 |
Redundancy | 20.3 | 18.5 | 17.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 3.2 M ammonium aulfate, 0.1 M Bis-Tris |