5W34
Crystal structure of the RNA polymerase domain (RPD) of Mycobacterium tuberculosis primase DnaG in complex with double-stranded DNA GACCGGAAGTGG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-07-20 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.781, 212.116, 47.418 |
Unit cell angles | 90.00, 107.87, 90.00 |
Refinement procedure
Resolution | 35.000 - 2.950 |
R-factor | 0.23829 |
Rwork | 0.236 |
R-free | 0.28532 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5w33 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.130 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.950 |
Number of reflections | 16383 |
<I/σ(I)> | 21 |
Completeness [%] | 94.6 |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 100 mM Hepes pH 7.0, 7% w/v PEG 4000 |