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5W34

Crystal structure of the RNA polymerase domain (RPD) of Mycobacterium tuberculosis primase DnaG in complex with double-stranded DNA GACCGGAAGTGG

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 22-ID
Synchrotron siteAPS
Beamline22-ID
Temperature [K]100
Detector technologyCCD
Collection date2016-07-20
DetectorRAYONIX MX300-HS
Wavelength(s)1.0
Spacegroup nameP 1 21 1
Unit cell lengths43.781, 212.116, 47.418
Unit cell angles90.00, 107.87, 90.00
Refinement procedure
Resolution35.000 - 2.950
R-factor0.23829
Rwork0.236
R-free0.28532
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)5w33
RMSD bond length0.007
RMSD bond angle1.130
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHASER
Refinement softwareREFMAC (5.8.0158)
Data quality characteristics
 Overall
Low resolution limit [Å]50.000
High resolution limit [Å]2.950
Number of reflections16383
<I/σ(I)>21
Completeness [%]94.6
Redundancy4.1
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP294100 mM Hepes pH 7.0, 7% w/v PEG 4000

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