5W2B

Crystal structure of C-terminal domain of Ebola (Reston) nucleoprotein in complex with Fab fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-14
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	47.508, 88.516, 137.166
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.623 - 2.250
R-factor	0.2007
Rwork	0.198
R-free	0.25380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vkd
RMSD bond length	0.007
RMSD bond angle	1.014
Data scaling software	HKL-3000
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.300
High resolution limit [Å]	2.250	2.250
Rpim	0.034	0.249
Number of reflections	28393	1835
<I/σ(I)>	3.21
Completeness [%]	99.8
Redundancy	7.3	5
CC(1/2)		0.836

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	288	20 mM xylitol, 20 mM myo-Inositol, 20 mM D-Fructose, 20 mM L-Rhamnose monohydrate, 20 mM D-Sorbitol, 0.1 M BES pH 7.5, 0.1 M triethanolamine (TEA) pH 7.5, 10% w/v PEG 8000, 20% w/v 1,5-Pentanediol