5W1M

MACV GP1 CR1-07 Fab complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 42 21 2
Unit cell lengths	206.970, 206.970, 238.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.120 - 3.910
R-factor	0.249
Rwork	0.248
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wfo
RMSD bond length	0.012
RMSD bond angle	1.330
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.120	49.120	4.150
High resolution limit [Å]	3.910	11.450	3.910
Rmerge	0.329	0.029	8.380
Rmeas	0.351	0.031	8.940
Total number of observations	378092
Number of reflections	46832	2013	7376
<I/σ(I)>	7.72	46.89	0.52
Completeness [%]	98.8	96.2	96.8
Redundancy	8.073	7.228	8.116
CC(1/2)	0.995	0.999	0.215

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	5.5	297	1.8 M AmSO4 and 100 mM NaCitrate pH 5.5.