5W1I

Crystal structure of LbaCas13a (C2c2) bound to mature crRNA (20-nt spacer)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.1158
Spacegroup name	P 1
Unit cell lengths	88.780, 89.212, 116.306
Unit cell angles	86.14, 86.97, 66.16

Refinement procedure

Resolution	48.420 - 2.200
R-factor	0.197
Rwork	0.197
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w1h
RMSD bond length	0.002
RMSD bond angle	0.415
Data reduction software	XDS
Data scaling software	Aimless (0.5.31)
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.570	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.110	1.060
Number of reflections	161267
<I/σ(I)>	8.6	1.3
Completeness [%]	97.5	96.6
Redundancy	3.6	3.7
CC(1/2)		0.466

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.2 M ammonium iodide, 20 - 25 % PEG 3,350