5W1C
Crystal structure of MBP fused activation-induced cytidine deaminase (AID) in complex with cytidine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-18 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 125.456, 39.824, 155.304 |
| Unit cell angles | 90.00, 90.27, 90.00 |
Refinement procedure
| Resolution | 125.455 - 3.180 |
| R-factor | 0.2341 |
| Rwork | 0.232 |
| R-free | 0.26990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5w0r |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.054 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 155.300 | 3.360 |
| High resolution limit [Å] | 3.180 | 3.180 |
| Rmerge | 0.543 | |
| Rpim | 0.335 | |
| Number of reflections | 28767 | 4138 |
| <I/σ(I)> | 8.9 | 2.5 |
| Completeness [%] | 99.3 | 99.7 |
| Redundancy | 6.5 | 6.9 |
| CC(1/2) | 0.974 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 289 | 0.1 M MES at pH 6.2, 3% PEG3350, 10 mM CaCl2 and 20mM Cytidine |
