5W0T
Crystal structure of monomeric Msp1 from S. cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-30 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 32 1 2 |
Unit cell lengths | 56.351, 56.351, 206.707 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 68.902 - 2.630 |
R-factor | 0.2317 |
Rwork | 0.230 |
R-free | 0.25960 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.550 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (dev_2499) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 206.710 | 2.700 |
High resolution limit [Å] | 2.630 | 2.630 |
Rmerge | 0.211 | 1.447 |
Rpim | 0.051 | 0.491 |
Number of reflections | 11608 | 843 |
<I/σ(I)> | 9.9 | 1.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 17.4 | 9.6 |
CC(1/2) | 0.990 | 0.762 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | ~9 mg/ml protein in 20 mM Hepes pH 7.5, 100 mM NaCl and 1 mM DTT was mixed with reservoir solution containing 16% PEG3350 and 0.6 M Sodium Thiocyanate |