5W0J
Antiparallel coiled coil hexamer formed by de novo peptides (ACC-Hex2).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 2 |
Unit cell lengths | 52.560, 52.560, 93.040 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.887 - 2.201 |
R-factor | 0.2253 |
Rwork | 0.220 |
R-free | 0.27690 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.761 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.890 | 2.290 |
High resolution limit [Å] | 2.201 | 2.201 |
Rmerge | 0.070 | 0.270 |
Rpim | 0.040 | 0.110 |
Number of reflections | 5328 | 300 |
<I/σ(I)> | 13.9 | 5.5 |
Completeness [%] | 99.5 | 100 |
Redundancy | 2 | 6.9 |
CC(1/2) | 0.999 | 0.960 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1 M sodium formate pH 6.5, 35% PEG 3350 |