5W0H
Structure of U2AF65 (U2AF2) RRM2 at 1.11 Angstrom Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-22 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9779 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.430, 38.203, 72.722 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.690 - 1.110 |
R-factor | 0.1537 |
Rwork | 0.153 |
R-free | 0.16810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2G4B RRM2 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.885 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.690 | 1.130 |
High resolution limit [Å] | 1.110 | 1.110 |
Rmerge | 0.081 | 0.278 |
Number of reflections | 36104 | |
<I/σ(I)> | 27.8 | 4.5 |
Completeness [%] | 97.2 | 92.2 |
Redundancy | 5.8 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 26% (w/v) PEG 8000, 0.1 M NaOAc, 0.2 mM TCEP, 10% (v/v) dioxane, 0.2 ul BigCHAP, 0.05 M potassium phosphate monobasic |