5W0G
Structure of U2AF65 (U2AF2) RRM1 at 1.07 resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-22 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9779 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 28.707, 28.707, 185.851 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 16.980 - 1.070 |
R-factor | 0.136 |
Rwork | 0.135 |
R-free | 0.15300 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2hzc |
RMSD bond length | 0.023 |
RMSD bond angle | 1.393 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX (1.11.1_2575) |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 16.980 | 1.090 |
High resolution limit [Å] | 1.070 | 1.070 |
Rmerge | 0.063 | 0.250 |
Number of reflections | 33973 | |
<I/σ(I)> | 16.3 | 2.9 |
Completeness [%] | 94.9 | 67.5 |
Redundancy | 4.5 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 21% (w/v) PEG MME 550, 210 mM zinc acetate, 100 mM MES |