5W0B
Structure of human TUT7 catalytic module (CM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.280 |
| Spacegroup name | P 61 |
| Unit cell lengths | 141.232, 141.232, 174.475 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 61.155 - 2.614 |
| R-factor | 0.2317 |
| Rwork | 0.231 |
| R-free | 0.25500 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.603 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.620 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.046 | 0.597 |
| Number of reflections | 59600 | |
| <I/σ(I)> | 32.8 | |
| Completeness [%] | 100.0 | |
| Redundancy | 9.3 | |
| CC(1/2) | 1.000 | 0.828 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.8-1.0 M lithium sulfate, 0.075-0.2 M potassium iodide, 0.05-0.2 M trisodium citrate, pH 5-6 |
