5W0A
Crystal structure of Trichoderma harzianum endoglucanase I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | BRUKER D8 QUEST |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-15 |
Detector | APEX II CCD |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.585, 99.325, 114.032 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.821 - 2.898 |
R-factor | 0.2382 |
Rwork | 0.234 |
R-free | 0.27440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1eg1 |
RMSD bond length | 0.020 |
RMSD bond angle | 1.409 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11rc2_2531: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.821 | 3.100 |
High resolution limit [Å] | 2.898 | 2.898 |
Rpim | 0.175 | 0.726 |
Number of reflections | 19374 | 3061 |
<I/σ(I)> | 5.3 | |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 19.7 | 13.5 |
CC(1/2) | 0.940 | 0.511 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | 0.1 M ammonium sulfate and 0.05 M Tris-HCl pH 7.5 |