5VZR
Crystal Structure of MERS-CoV neutralizing antibody G4 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-27 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.97926 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 80.678, 88.759, 128.469 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 73.025 - 1.570 |
R-factor | 0.1839 |
Rwork | 0.182 |
R-free | 0.21510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDBIDs 2JEL and 3QQ9 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.849 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless (0.5.21) |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 88.760 | 88.760 | 1.600 |
High resolution limit [Å] | 1.570 | 8.600 | 1.570 |
Rmerge | 0.128 | 0.064 | 1.641 |
Total number of observations | 786682 | 5660 | 40011 |
Number of reflections | 128851 | ||
<I/σ(I)> | 7.8 | 19.3 | 1.7 |
Completeness [%] | 99.8 | 100 | 99.8 |
Redundancy | 6.1 | 6.2 | 6.3 |
CC(1/2) | 0.994 | 0.951 | 0.601 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M MES pH 6.5, 0.2 M magnesium chloride and 10% (w/w) PEG4000 |