5VZH
Post-catalytic complex of human Polymerase Mu (W434H) mutant with incoming UTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-07 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.637, 68.722, 109.404 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.042 - 1.950 |
R-factor | 0.1805 |
Rwork | 0.179 |
R-free | 0.21080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m04 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.819 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Rpim | 0.023 | 0.332 |
Number of reflections | 33554 | 1620 |
<I/σ(I)> | 22.7 | 2.01 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 7 | 4.2 |
CC(1/2) | 0.771 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 90mM HEPES pH 7.5, 18% PEG4K |