5VZB

Post-catalytic complex of human Polymerase Mu (G433S) mutant with incoming UTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-13
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	59.807, 68.843, 109.848
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.328 - 1.500
R-factor	0.1716
Rwork	0.171
R-free	0.18740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
RMSD bond length	0.009
RMSD bond angle	1.075
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.530
High resolution limit [Å]	1.500	1.500
Rpim	0.027	0.339
Number of reflections	72904	3437
<I/σ(I)>	25.34	2.45
Completeness [%]	99.5	95.8
Redundancy	6.8	3.5
CC(1/2)		0.688

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	90mM HEPES pH 7.5, 18% PEG4K