5VZ9

Post-catalytic complex of human Polymerase Mu (G433A) mutant with incoming dTTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-13
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	60.190, 68.847, 111.355
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.145 - 1.650
R-factor	0.1619
Rwork	0.160
R-free	0.19050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
RMSD bond length	0.009
RMSD bond angle	1.001
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.680
High resolution limit [Å]	1.650	1.650
Rpim	0.030	0.369
Number of reflections	56900	2827
<I/σ(I)>	27.4	3.14
Completeness [%]	99.9	100
Redundancy	7.1	5.4
CC(1/2)		0.806

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	50mM HEPES pH 7.5, 10% PEG4K