5VZ8

Post-catalytic complex of human Polymerase Mu (G433A) mutant with incoming UTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-13
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	59.934, 69.031, 109.825
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.910 - 1.601
R-factor	0.1729
Rwork	0.172
R-free	0.19360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
RMSD bond length	0.009
RMSD bond angle	1.077
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.630
High resolution limit [Å]	1.600	1.600
Rpim	0.030	0.344
Number of reflections	59923	2914
<I/σ(I)>	24.9	3.1
Completeness [%]	98.7	97.6
Redundancy	7.2	5.7
CC(1/2)		0.769

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	50mM HEPES pH 7.5, 10% PEG4K