5VYV
Crystal structure of a protein of unknown function YceH/ECK1052 involved in membrane biogenesis from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2017-05-24 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.777, 74.853, 58.661 |
Unit cell angles | 90.00, 100.73, 90.00 |
Refinement procedure
Resolution | 28.817 - 2.480 |
R-factor | 0.2094 |
Rwork | 0.206 |
R-free | 0.26550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5u8j |
RMSD bond length | 0.003 |
RMSD bond angle | 0.610 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((dev_2733: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.520 |
High resolution limit [Å] | 2.480 | 2.480 |
Rmerge | 0.092 | 0.806 |
Rpim | 0.048 | 0.435 |
Number of reflections | 11477 | 565 |
<I/σ(I)> | 21.19 | 2.08 |
Completeness [%] | 100.0 | 100 |
Redundancy | 4.6 | 4.4 |
CC(1/2) | 0.631 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 20% PEG 8K, 50 mM potassium dihydrogen phosphate |