5VYM
Crystal structure of beta-galactosidase from Bifidobacterium adolescentis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-01 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.37762 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 90.549, 90.549, 278.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.110 - 2.456 |
| R-factor | 0.1852 |
| Rwork | 0.181 |
| R-free | 0.22630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uzs |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.420 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (dev_2650) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 36.110 | 50.000 | 2.490 |
| High resolution limit [Å] | 2.450 | 6.650 | 2.450 |
| Rmerge | 0.076 | 0.033 | 0.786 |
| Rmeas | 0.081 | 0.036 | 0.832 |
| Rpim | 0.027 | 0.013 | 0.264 |
| Total number of observations | 205921 | ||
| Number of reflections | 36438 | ||
| <I/σ(I)> | 8.6 | ||
| Completeness [%] | 98.9 | 96.7 | 100 |
| Redundancy | 8.1 | 6.8 | 9 |
| CC(1/2) | 0.997 | 0.856 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 297 | 0.2M Lithiumsulfate, 0.1M Sodium acetate, 30% PEG8000 |
