5VYM

Crystal structure of beta-galactosidase from Bifidobacterium adolescentis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-01
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.37762
Spacegroup name	P 61 2 2
Unit cell lengths	90.549, 90.549, 278.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.110 - 2.456
R-factor	0.1852
Rwork	0.181
R-free	0.22630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uzs
RMSD bond length	0.002
RMSD bond angle	0.420
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX (dev_2650)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.110	50.000	2.490
High resolution limit [Å]	2.450	6.650	2.450
Rmerge	0.076	0.033	0.786
Rmeas	0.081	0.036	0.832
Rpim	0.027	0.013	0.264
Total number of observations	205921
Number of reflections	36438
<I/σ(I)>	8.6
Completeness [%]	98.9	96.7	100
Redundancy	8.1	6.8	9
CC(1/2)		0.997	0.856

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	297	0.2M Lithiumsulfate, 0.1M Sodium acetate, 30% PEG8000