5VYL
Crystal Structure of N-terminal half of Herpes Simplex virus Type 1 UL37 protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979180 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 138.232, 138.232, 164.220 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 96.737 - 3.510 |
R-factor | 0.2442 |
Rwork | 0.240 |
R-free | 0.28160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4k70 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.933 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 164.220 | 3.850 |
High resolution limit [Å] | 3.510 | 3.510 |
Rmerge | 0.441 | 1.993 |
Rpim | 0.119 | 0.539 |
Number of reflections | 12161 | 2818 |
<I/σ(I)> | 8.7 | 1.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 14 | 14.1 |
CC(1/2) | 0.993 | 0.580 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | 0.1M Bicine pH 9.0, 8% PEG 20K |