5VYJ
Crystal structure of the photosynthetic phosphoenolpyruvate carboxylase isoenzyme from maize in complex with Gly
Replaces: 4UOLExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-20 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 156.950, 167.240, 242.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.079 - 3.300 |
R-factor | 0.2118 |
Rwork | 0.210 |
R-free | 0.25270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jqo |
RMSD bond length | 0.007 |
RMSD bond angle | 1.001 |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.080 | 3.420 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.120 | 0.440 |
Number of reflections | 90116 | |
<I/σ(I)> | 11.2 | 2.8 |
Completeness [%] | 93.2 | 62.2 |
Redundancy | 3.9 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291.15 | 0.2 M SODIUM ACETATE TRIHYDRATE, 0.1 M TRIS HYDROCHLORIDE PH 8.5, 15% (W/V) PEG 4000, 0.1M POTASSIUM/SODIUM TARTRATE-4-HYDRATE |