5VX5
VP8* of a G2P[4] Human Rotavirus in complex with LNFP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-09-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9774 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.790, 50.240, 110.820 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.040 - 1.285 |
R-factor | 0.1537 |
Rwork | 0.153 |
R-free | 0.16720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2aen |
RMSD bond length | 0.006 |
RMSD bond angle | 1.104 |
Data reduction software | iMOSFLM (7.0.9) |
Data scaling software | SCALA (3.3.21) |
Phasing software | PHASER (2.5.5) |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.040 | |
High resolution limit [Å] | 1.285 | 1.285 |
Rmerge | 0.056 | 0.269 |
Rpim | 0.022 | 0.102 |
Number of reflections | 89379 | |
<I/σ(I)> | 19.58 | |
Completeness [%] | 93.4 | |
Redundancy | 2 | |
CC(1/2) | 0.999 | 0.963 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.2 M sodium acetate, 0.1 M Tris, pH 8.5, 30% w/v PEG4000 |