5VX4

VP8* of a G2P[4] Human Rotavirus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-09-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9774
Spacegroup name	P 1 21 1
Unit cell lengths	34.330, 56.590, 37.310
Unit cell angles	90.00, 93.52, 90.00

Refinement procedure

Resolution	31.110 - 1.550
R-factor	0.1734
Rwork	0.171
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2aen
RMSD bond length	0.007
RMSD bond angle	1.050
Data reduction software	iMOSFLM (7.0.9)
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER (2.5.5)
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.110
High resolution limit [Å]	1.550	1.550
Rmerge	0.025	0.228
Rmeas	0.025
Rpim	0.025	0.122
Number of reflections	19627
<I/σ(I)>	18.61
Completeness [%]	94.5
Redundancy	2
CC(1/2)	0.999	0.954

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	0.1 M Bis-Tris propane, pH 9.0, 30% w/v PEG6000