5VWK
Crystal structure of human Scribble PDZ1:Beta-PIX complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-09-03 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9537 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 74.760, 74.760, 222.963 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.357 - 2.350 |
R-factor | 0.2245 |
Rwork | 0.223 |
R-free | 0.25650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2w4f |
RMSD bond length | 0.004 |
RMSD bond angle | 0.656 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.770 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.126 | 0.738 |
Number of reflections | 26459 | |
<I/σ(I)> | 7.3 | 1.8 |
Completeness [%] | 97.5 | 99.2 |
Redundancy | 4.1 | 4.1 |
CC(1/2) | 0.996 | 0.738 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.09 | 293 | 1.09M lithium sulfate and 0.1M trisodium citrate-citric acid pH5.09 |