5VWK

Crystal structure of human Scribble PDZ1:Beta-PIX complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-03
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9537
Spacegroup name	P 43 21 2
Unit cell lengths	74.760, 74.760, 222.963
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.357 - 2.350
R-factor	0.2245
Rwork	0.223
R-free	0.25650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2w4f
RMSD bond length	0.004
RMSD bond angle	0.656
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.770	2.430
High resolution limit [Å]	2.350	2.350
Rmerge	0.126	0.738
Number of reflections	26459
<I/σ(I)>	7.3	1.8
Completeness [%]	97.5	99.2
Redundancy	4.1	4.1
CC(1/2)	0.996	0.738

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.09	293	1.09M lithium sulfate and 0.1M trisodium citrate-citric acid pH5.09