5VWI

Crystal structure of human Scribble PDZ1:Beta-PIX complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-20
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	37.869, 44.163, 60.465
Unit cell angles	90.00, 93.61, 90.00

Refinement procedure

Resolution	31.160 - 1.750
R-factor	0.1983
Rwork	0.194
R-free	0.23560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wyt
RMSD bond length	0.011
RMSD bond angle	1.195
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.160	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.072	1.759
Number of reflections	20310
<I/σ(I)>	17.6	1
Completeness [%]	99.8	99.9
Redundancy	6.2	6.1
CC(1/2)	0.999	0.307

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.9	293	16.6% (w/v) polyethylene glycol 3350 and 0.1M citrate-bis-tris propane pH8.9