5VWG
Galectin-8 N terminal domain in complex with Methyl 3-O-[1-carboxyethyl]-beta-D-galactopyranoside
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 298 |
Detector technology | PIXEL |
Collection date | 2016-06-10 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.777, 50.145, 80.695 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.900 - 2.100 |
R-factor | 0.2217 |
Rwork | 0.220 |
R-free | 0.25084 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5t7u |
RMSD bond length | 0.007 |
RMSD bond angle | 1.307 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.900 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.149 | 0.782 |
Number of reflections | 11077 | 873 |
<I/σ(I)> | 5.9 | 1.9 |
Completeness [%] | 98.0 | 95 |
Redundancy | 4.9 | 4.8 |
CC(1/2) | 0.981 | 0.546 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277 | 10 mM sodium phosphate, 137 mM sodium chloride, 2.7 mM potassium chloride, 1.8 mM potassium phosphate |