5VWF
HLA-B*58:03 presenting LTVQVARVY
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.954 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.759, 81.704, 109.495 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.850 - 1.800 |
R-factor | 0.159 |
Rwork | 0.157 |
R-free | 0.19600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rfx |
RMSD bond length | 0.019 |
RMSD bond angle | 1.847 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.852 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.140 | 0.710 |
Number of reflections | 42970 | |
<I/σ(I)> | 9.5 | 3.4 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 5.4 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 298 | 12 -20 % PEG 4000, 0.2 M ammonium acetate and 0.1 M tri-sodium citrate pH 5.4 |