5VWB
Y316F mutant of corn root ferredoxin:NADP+ reductase in alternate space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 140 |
Detector technology | IMAGE PLATE |
Collection date | 2006-11-20 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.542 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 59.306, 59.306, 186.221 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.834 - 1.800 |
R-factor | 0.1597 |
Rwork | 0.154 |
R-free | 0.21180 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.186 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.834 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.062 | 0.587 |
Number of reflections | 32137 | 1058 |
<I/σ(I)> | 10.4 | 1 |
Completeness [%] | 88.6 | 30 |
Redundancy | 4.4 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 22-24% PEG 8000, 0.1 M sodium cacodylate (pH 6-7), 0.18-0.22 M magnesium acetate, 100 mM nicotinamide |