5VWB
Y316F mutant of corn root ferredoxin:NADP+ reductase in alternate space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 140 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-11-20 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 59.306, 59.306, 186.221 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.834 - 1.800 |
| R-factor | 0.1597 |
| Rwork | 0.154 |
| R-free | 0.21180 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.186 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.834 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.062 | 0.587 |
| Number of reflections | 32137 | 1058 |
| <I/σ(I)> | 10.4 | 1 |
| Completeness [%] | 88.6 | 30 |
| Redundancy | 4.4 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 22-24% PEG 8000, 0.1 M sodium cacodylate (pH 6-7), 0.18-0.22 M magnesium acetate, 100 mM nicotinamide |
