5VW9
Nicotinamide soak of Y316S mutant of corn root ferredoxin:NADP+ reductase in alternate space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 140 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-10-17 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 58.900, 58.900, 184.407 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 11.478 - 1.894 |
| R-factor | 0.1354 |
| Rwork | 0.131 |
| R-free | 0.17320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lo8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.056 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 11.480 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.040 | 0.153 |
| Number of reflections | 29752 | 2603 |
| <I/σ(I)> | 19.4 | 6.9 |
| Completeness [%] | 97.6 | 86.6 |
| Redundancy | 8.2 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 22-24% PEG 8000, 0.1 M sodium cacodylate (pH 6-7), 0.18-0.22 M magnesium acetate, 100 mM nicotinamide |
