5VW8
NADP+ soak of Y316S mutant of corn root ferredoxin:NADP+ reductase in alternate space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 140 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-01-12 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 58.867, 58.867, 185.036 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.295 - 1.808 |
| R-factor | 0.1757 |
| Rwork | 0.172 |
| R-free | 0.20980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lo8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.272 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.300 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.101 | 0.243 |
| Number of reflections | 35032 | 5039 |
| <I/σ(I)> | 6.5 | 2.2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.3 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 22-24% PEG 8000, 0.1 M sodium cacodylate (pH 6-7), 0.18-0.22 M magnesium acetate, 100 mM nicotinamide |
