5VW7
NADPH soak of Y316A mutant of corn root ferredoxin:NADP+ reductase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 59.188, 59.188, 188.032 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.454 - 1.608 |
R-factor | 0.1332 |
Rwork | 0.131 |
R-free | 0.15560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lo8 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.170 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.450 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.028 | 0.165 |
Number of reflections | 50821 | 4336 |
<I/σ(I)> | 24.5 | 6 |
Completeness [%] | 98.6 | 86.4 |
Redundancy | 4 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 22-24% PEG 8000, 0.1 M sodium cacodylate (pH 6-7), 0.18-0.22 M magnesium acetate, 100 mM nicotinamide |