5VW2
NADPH soak of Y316S mutant of corn root ferredoxin:NADP+ reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-02 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 58.849, 58.849, 187.301 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.960 - 1.451 |
| R-factor | 0.114 |
| Rwork | 0.110 |
| R-free | 0.14650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lo8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.960 | 1.530 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.036 | 0.238 |
| Number of reflections | 66408 | 8907 |
| <I/σ(I)> | 12.7 | 2.7 |
| Completeness [%] | 98.1 | 92.1 |
| Redundancy | 8.4 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 22-24% PEG 8000, 0.1 M sodium cacodylate (pH 6-7), 0.18-0.22 M magnesium acetate, 100 mM nicotinamide |
