5VVM

Crystal structure of human CDH23 EC21-23

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU MICROMAX-003
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-04
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	116.714, 166.806, 47.930
Unit cell angles	90.00, 102.53, 90.00

Refinement procedure

Resolution	94.080 - 3.540
R-factor	0.305
Rwork	0.303
R-free	0.34600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5i8d 5uz8
RMSD bond length	0.010
RMSD bond angle	1.349
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.080	3.610
High resolution limit [Å]	3.540	3.540
Rmerge	0.160	0.425
Number of reflections	10856
<I/σ(I)>	5.95	2.2
Completeness [%]	99.4	99.6
Redundancy	2.9	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.05 M TRIS pH 8.5 0.5 M NaCl 10% PEG-4000