5VVF

Crystal Structure of 354BG1 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-10
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	95.630, 95.630, 103.951
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.970 - 2.000
R-factor	0.214
Rwork	0.210
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2909 FAB (PDB CODE 3PIQ)
RMSD bond length	0.007
RMSD bond angle	0.948
Data reduction software	Aimless (7.0.038)
Data scaling software	Aimless (7.0.038)
Refinement software	PHENIX ((1.11_2567: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	82.820
High resolution limit [Å]	2.000
Number of reflections	37475
<I/σ(I)>	7.9
Completeness [%]	99.7
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	1.8 M ammonium sulfate, 0.1 M Bis-Tris pH 6.5, 2% v/v PEG 550 MME, 100 nM NDSB-256