5VU7

TNA polymerase, open ternary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-18
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 2 21 21
Unit cell lengths	66.550, 112.610, 145.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.098 - 2.720
R-factor	0.218
Rwork	0.214
R-free	0.26770
RMSD bond length	0.003
RMSD bond angle	0.624
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.100	2.790
High resolution limit [Å]	2.720	2.720
Rmerge	0.074	0.575
Number of reflections	29340
<I/σ(I)>	7.6
Completeness [%]	97.4
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	0.2 M sodium sulfate, 0.1 M MES, pH 5.5, 3% w/v 1,6-hexanediol, 22% PEG3350